Question 1:
GEAC stands for
Question 2:
RNA interference (RNAi) helps in making tobacco-plant resistant to a nematode (Meloidegyne incognitia)
Choose the correct option that shows how RNAi is achieved :
Question 3:
Which one of the following is not the product of transgenic experiments?
Question 4:
Out of the following, select the correct match:
Question 5:
**Assertion (A):** In molecular diagnosis, single stranded DNA or RNA tagged with radioactive molecule is called a probe.
**Reason (R):** A probe always searches and hybridises with its complementary DNA in a clone of cells.
**Reason (R):** A probe always searches and hybridises with its complementary DNA in a clone of cells.
Question 6:
A patient with ADA deficiency requires periodic infusion of genetically engineered lymphocytes. Explain why such periodic infusion is required and also suggest a permanent cure for such ADA deficiency.
Question 7:
(a) Name the Indian crop variety for which, in 1997, an American company got patent rights through the US Patent and Trademark Office. This is considered a case of Biopiracy. Justify.
OR
(b) State two purposes for which the Indian Government has set up GEAC (Genetic Engineering Approval Committee).
OR
(b) State two purposes for which the Indian Government has set up GEAC (Genetic Engineering Approval Committee).
Question 8:
(a) The insulin synthesised in our body is different from that synthesised by Eli Lilly company using recombinant DNA technology. Differentiate between them.
(b) Why the insulin extracted from an animal source is not in use these days?
(b) Why the insulin extracted from an animal source is not in use these days?
Question 9:
- (a) Why are transgenic animals so called?
- (b) With the help of an example each, explain the role of transgenic animals in the following:
- (i) Vaccine safety
- (ii) Biological products
Question 10:
Explain the beneficial role of the following, produced as a result of the processes of biotechnology, to mankind :
- (a) Cow named Rosie
- (b) $\alpha-1$-antitrypsin
Question 11:
(a) What are transgenic animals ?
(b) Name the transgenic animal having the largest number amongst all the existing transgenic animals.
(c) State any 3 reasons for which these types of animals are being produced.
(b) Name the transgenic animal having the largest number amongst all the existing transgenic animals.
(c) State any 3 reasons for which these types of animals are being produced.
Question 12:
(a) (i) Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
(ii) How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists ?
OR
(b) (i) Explain how the amplification of gene of interest is done using PCR.
(ii) State two applications of the desired amplified fragment of DNA.
(ii) How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists ?
OR
(b) (i) Explain how the amplification of gene of interest is done using PCR.
(ii) State two applications of the desired amplified fragment of DNA.
Question 13:
(a) Name and explain the biotechnological strategy wherein the infection by the nematode Meloidegyne incognitia can be prevented using Agrobacterium vectors in the roots of tobacco plant by RNA interference.
OR
(b) Explain the amplification of gene of interest using the technique of Polymerase chain reaction (PCR).
OR
(b) Explain the amplification of gene of interest using the technique of Polymerase chain reaction (PCR).
Question 14:
**OR**
(i) Name the nematode (scientific name) that infects the roots of tobacco plant and reduces its yield.
(ii) Name the vector that is used to introduce nematode-specific genes into the host plant (tobacco).
(iii) How do sense and anti-sense RNAs function ?
(iv) Why could parasite not survive in a transgenic tobacco plant ?
(i) Name the nematode (scientific name) that infects the roots of tobacco plant and reduces its yield.
(ii) Name the vector that is used to introduce nematode-specific genes into the host plant (tobacco).
(iii) How do sense and anti-sense RNAs function ?
(iv) Why could parasite not survive in a transgenic tobacco plant ?